show Abstracthide AbstractThe purpose of this study was to investigate the role of the yeast protein, high mobility group protein (Hmo1), in rRNA synthesis. For over twenty years, Hmo1 has been proposed to be an activator of transcription by Pol I, but its regulatory mechanism remains largely undefined. Therefore, we used native elongating transcript sequencing (NET-seq) to explore the role of this factor in transcription by Pol I. Our NET-seq results demonstrated that in cells lacking Hmo1 (hmo1D), Pol I occupancy was significantly increased across the rDNA template, indicating an increased pause propensity in these cells. These data suggest that Hmo1 is an important transcription elongation factor for Pol I. Overall design: Triplicate NET-seq libraries were generated for wild-type (WT) and hmo1? yeast strains. Please note that the WT samples (in GSE216460/PRJNA893665) were re-analysed in the current study: GEO accession title SRA accessions Biosample accession ========= ==== ========== ============= GSM6674347 WT yeast, untreated, rep1 SRR22028427/SRX18009711 SAMN31430711 GSM6674348 WT yeast, untreated, rep2 SRR22028426/SRX18009712 SAMN31430710 GSM6674349 WT yeast, untreated, rep3 SRR22028425/SRX18009713 SAMN31430709